Regulation of adult skeletal muscle stem cell behaviour

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theoretical investigation of the potential role of the gene sequence provided in adult skeletal muscle stem cell behaviour”

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provided cDNA sequence: TCTAGGTCGACGGTNTCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCTCTATATGTTTTGATGGGGCTGCACATCATCTTGTCAGGTCCTGTCATTGAAAAGATGGCTCTCAGCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGTTCTCGCTCGGATGAGCTTACCCGCCATATCCGCATCCACACAGGCCAGAAGCCCTTCCAGTGTCGAATCTGCATGCGTAACTTCAGTCGTAGTGACCACCTTACCACCCACATCCGCACCCACACNGGCGAGAAGCCTTTTGCCTGTGACATTTGTGGGAGGAAGTTTGCCAGGANTGATGANNGNAAGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTCGCGCAGCGTTGACCNNTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTTCGCNTTTCTTCCCTTCCTTTCTCGNCACGT

  • Describes the aim of the project and summarizes the essence of the underlying hypothesis
  • Describes strategy and methodology
  • Describes relevant techniques and skillsdeveloped.
  • Describes results and conclusion clearly
  • Makes a connection of developed skill
  1. What is the sequence?
    NB. Sometimes, the sequence contains more than one fragment that ligated together during the cloning procedure prior to sequencing
    – Is the sequence ALL one transcript?
    – Is there any genomic sequence?
  1. What is the structure of the transcript (introns/exons)
    Is there more than one splice variant?
    Is it part of a family?
  1. What is the protein product?
    – Are there any functional domains?
    – What do these domains do?
  1. What pathway(s)/functions does this gene product play a role in?
  1. What is known about the expression patterns of the gene (during development and in the adult)
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